Novatia : Capillary NMR of Brucine

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Capillary NMR of Brucine

Author: David Detlefsen

Posted: 3/13/03; 10:43:21 AM

Topic: Capillary NMR of Brucine

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Looking for more examples of CapNMR capabilities? You've come to the right place. On this page we'll list some sample data we've collected to illustrate the high sensitivity and high quality of the NMR data that can be obtained on trace amounts of material. All data (unless otherwise specified) were collected using MRM's CapNMR probe on a Varian 600 MHz Inova Spectrometer.

Brucine: Brucine is an analog of strychnine (two methoxy groups on aromatic ring). It's also know as 10,11-dimethoxystrychnine. Brucine has a molecular formula of C23H26N2O4 and a molecular weight of 394.45. It's highly soluble in chloroform. We prepared a sample of approximately 50 mM brucine for our studies. We injected a total volume of 13 ul (5 ul in the CapNMR detection cell) using Novatia's Manual Injection CapNMR Plumbing Pak. The corresponds to about 50 ug of Brucine in the detection cell (or about 127 nMoles).

brucine_structure: Chemical Structure of Brucine.

1D 1H of Brucine
50 ug
20 seconds
4 scans brucine_50ug_1D1H: Brucine 1D 1H spectrum of 50 ug at 600 MHz.

2D 1H gCOSY of Brucine
50 ug
5 minutes
1 X 256 T1 Increments †brucine_50ug_gcosy: Brucine 50 ug gCOSY by Capillary NMR at 600 MHz in 5 minutes.

2D 1H -13C HMQC of Brucine
50 ug
1.5 hours
16 X 128 T1 Increments brucine_50ug_hmqc: 1H-13C HMQC of 50ug Brucine in 1.4 hours by Capillary NMR at 600 MHz.

2D 1H -13C gHMBC of Brucine
50 ug
4 hours
48 X 256 T1 Increments brucine_50ug_hmbc: