Novatia Sample Submission Form
To submit an analysis request, begin by downloading a blank routine sample submission sheet. Open the blank sheet in Microsoft Excel and enter the sample information and details of the analysis requested for each sample to be submitted. Fields requiring information will be highlighted on the Excel sheet. Please include comments about samples on the submission on the Excel sheet. Please do this by using the comments section (column N) of the Excel sample submission sheet that you are uploading. The sample submission sheet will tabulate the analysis requests and calculate the estimated cost. Save the sheet and proceed with Steps 1 through 4. Select the Submit button at the bottom of the page and a confirmation will be presented summarizing the request with shipping instructions. A confirmation will also be emailed to the contact entered in Step 3. (Check your spam folder if you have not received a confirmation email)
- Rush Services available for 100% additional cost
- See Novatia website for detailed pricing information
NMR Sample Requirements:
- 1D Standard Analysis: >1 mg
- 1D Standard 13C Analysis: >50 mg
- 2D Standard Analysis: >10 mg
- Samples must be received in either a sealed vial or sealed Eppendorf tube. Solid samples received in cellophane or zip lock bags will not be accepted and new samples provided in the acceptable storage containers will be requested.
NMR Analysis Time:
- Analysis results usually within 5 business days of sample receipt
NMR Results:
- Standard PDF Plots with peak list and integration
- Raw data available upon request
NMR Experiment Key:
- Proton = Hydrogen
- F_19 = Fluorine
- P_31 = Phosphorus
- C_13 = Carbon
- C_13_DEPT = DEPT Carbon
- STAN_1H_COSY_HSQC_HMBC = 2D analysis including 1D proton, COSY, HSQC, and HMBC
- Rush Services available for 100% additional cost
- See Novatia website for detailed pricing information
Note: MW Confirmation refers to nominal mass only
MS Sample Requirements
- Minimum sample amounts
- Small Molecules
- 50-100 ug (e.g. 1-2 mg/mL x 25-50 uL)
- Ideal for small molecule routine work would be at least 50 microliters of 400 micromolar sample.
- Small molecule samples must be received in either a sealed vial or sealed Eppendorf tube. Solid samples received in cellophane or zip lock bags will not be accepted and new samples provided in the acceptable storage containers will be requested.
- Oligonucleotides
- Submit > 1 nmol if sending an oligonucleotide for oligo sequencing
- Submit > 50 pico moles for oligo analyses. An ideal oligo submission for routine work would be at least 30 microliters of 5 micromolar sample
- Proteins/ Peptides
- 50-100 ug (e.g. 2 mg/ mL x 25-50 uL) for peptide mapping
- 20-40 ug (e.g. 2 mg/mL x 10-20 uL) for Native MS methods
- 10-20 ug (e.g. 1 mg/mL x 10-20 uL) for all other protein/peptide analysis.
- Small Molecules
- Samples can be dry or lyophilized sample (specify grams or moles) or in solution (specify volume & concentration)
- If the samples contain any detergent (Tween, TritonX, etc) tell us in the comments and provide an estimated concentration.
- Provide target molecular weight, molecular formula, or structure.
- Oligonucleotide sequences (use IDT designation as specified at IDT Oligo Nomenclature) this includes sequence modifications if they are available. If the modification is unique please provide a molecular formula or structure.
- If submitting a small molecule, please indicate the structure and/or functional groups, a solvent, and any other important information in the Comments field of the Excel sheet
Plate Submission Notes:
Discount Pricing is only available for the following MS methods:
- Oligonucleotide MW_Confirmation
- Oligonucleotide LCMS_5_Min
To Qualify for Discount Pricing:
- Samples MUST be in a skirted plate with conical/V-shape wells. See below for accepted plates.
- Plate position for each sample must be specified in Excel submission sheet.
- All samples must be at concentrations between 5 uM – 10 uM with a minimum of 100 uL of sample.
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- If plate is shipped dried-down, 0.5-1 nanomole of sample is required
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- Uniform MS method selected for all samples on the submission sheet.
If these requirements are not met, the submission will be placed on a HOLD and the contact for that submission will be reached out. If the samples are received in an ineligible plate, we can “re-plate” the samples for a $250 surcharge. For issues regarding other requirements, we will confirm whether to proceed with the requested analysis with full pricing per sample.
Plate Shipping & Accepted Plates Information:
- Plates are subject to review to qualify for discount pricing when received. Example of accepted 96-Well Plates are below:
- https://www.thermofisher.com/order/catalog/product/A31728
- https://www.thermofisher.com/order/catalog/product/249943
- https://www.waters.com/nextgen/us/en/shop/vials-containers–collection-plates/wat058957-96-well-sample-collection-plate-1-ml-50-pk.html
- https://www.waters.com/nextgen/us/en/shop/vials-containers–collection-plates/186005837-96-well-sample-collection-plate-700–l-round-well-25-pk.html
- If you are not using one of the plates listed above, we recommend you contact us to confirm if your plate is acceptable for discount pricing prior to submitting.
- To prevent cross-contamination and potential sample loss, it is best to ship them either on dry ice, or dried-down.
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- Even the best plate seals it is likely that cross-contamination will happen during the shipping process.
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MS Analysis Time:
- MW Confirmation usually within 1 business day of sample receipt
- Purity Profile usually within 2 business days of sample receipt
- High Resolution data usually within 1-5 business days of sample receipt
- Large quantities of samples may take longer
MS Results:
- Web-based ProMass report available at our secure data server
- Click here to see an example of a ProMass report