Oligonucleotide Sequence Confirmation
…Using High Resolution Ion Trap Tandem Mass Spectrometry
It is important to confirm the structure of oligonucleotides to be used in diagnostic and therapeutic applications. The use of modified oligonucleotides often precludes the use of enzymatic digestion combined with mass spectrometry for sequence confirmation. Tandem mass spectrometry can be used to reliably confirm the sequence of DNA and RNA, including modified oligos. Applications include sequence confirmation of known sequences, as well as denovo sequencing and identification of modified residues.
Novatia uses a unique approach that utilizes high resolution hybrid LTQ-Orbitrap mass spectrometry and novel deisotoping and charge deconvolution software for MS-based oligonucleotide sequencing. For more detailed information…