Research

SEQUEST tips

Running SEQUEST:

• How to evaluate SEQUEST results
• How to use SEQUEST to search a nucleotide database
• Error messages when clicking on database reference on the SEQUEST summary page
• Fixes for known SEQUEST problems.  If you think you have found a problem with SEQUEST/TurboSEQUEST, there may be a fix.  Go here to see if a recent solution is available.

SEQUEST Browser Tips:

• Running SEQUEST from Qual Browser vs. SEQUEST Browser
• How to change the SEQUEST browser data directory - Contrary to popular belief, you can change where the SEQUEST browser looks for raw data files.  Here's how.
• Sequest Browser Run Summary Bug Fix - Fix a bug that affects some of the SEQUEST browser controls with Internet Explorer.
• Configuring the SEQUEST Browser Server - Go here to make sure your server software is configured properly.  
• Securing your SEQUEST Browser - Learn how to change your web server properties to add security to your SEQUEST browser site.
• Interactive SEQUEST Browser Summary Page - Confused about SEQUEST output?  Study our interactive clickable summary page and you'll be a SEQUEST captain in no time.

For more information check out:

Top 10 hits for SEQUEST cross-correlation on.. 1. MS/MS Database Searching 2. Selective Detection of Membrane Proteins Without Antibodies: A ... 3. Novatia : SEQUEST tips 4. ProtQuant: a tool for the label-free quantification of MudPIT ... 5. Developmental & Comparative Immunology : Characterization and cDNA ... 6. BioMed Central | Full text | ProtQuant: a tool for the label-free ... 7. Immobilized pH Gradient Isoelectric Focusing as a First-Dimension ... 8. Protein identification: a look into the black box 9. Oncogene - Direct cancer tissue proteomics: a method to identify ... 10. A statistical framework for genomic data fusion  5/9/08; 9:19:55 AM.

Here's a list of all SEQUEST-related messages on this site. the SEQUEST site at Scripps the SEQUEST patent the original SEQUEST manuscript: "An Approach to Correlate Tandem Mass Spectral Data of Peptides with Amino Acid Sequences in a Protein Database", Eng, J.; McCormack, A.L.; Yates, J.R., III. J. Am. Soc. Mass Spectrom. 1994, 5, 976-989. a good general review article: "Mining Genomes with MS", Yates, J.R., III.; McCormack, A.L.; Eng, J.; Anal. Chem.1996, 68(17), 534A-540A. Add ProMass automated biomolecular weight determination to your proteomics capabilities!

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