Metabolism

Novatia offers a wide range of services to support your metabolite studies, in areas as diverse as Screening, Profiling, and Identification, on samples from In-Vitro or In-Vivo origin, using both Nominal and Accurate Mass instrumentation.

Metabolism Screening Assays

Novatia offers high-throughput in-vitro data of drug Metabolic Stability, Half-Life Determination, P450 Enzyme Specificity, etc. We provide non-cellular (microsomes, S9, cytosol, etc.) and cellular assays (fresh and cryopreserved hepatocytes from multiple species). All analyses are performed using high-throughput LC/MS/MS methods for speed, sensitivity, and specificity. We also encourage assay customization which offers the best chance of integrating our results into your process. We have automated data processing routines in place to provide further ease of data reporting. Customized reporting can also be accommodated to meet your specific needs.

Metabolite Profiling

It is highly desirable to obtain simultaneous identification of drug metabolites along with component profiling information for inter-species comparisons. By acquiring the full scan and MS/MS data during an LC/MS run, we are able to propose sites of biotransformation for most major metabolites and relate their relative abundance across samples. Rather than conduct slow manual interpretation at this stage, we quickly provide this time-sensitive data using our own patented software, MetLab Profiler (Bottom Up), along with custom Metabolomics processing tools (Top Down). This data processing approach leverages current MetID tools for finding parent related components, while at the same time, minimizes the possibility of missing unique and unpredictable metabolites. We can provide you the critical data, for your chemistry efforts by finding metabolic “hot-spots” in your lead series, right up to determining highly detailed biotransformation profiles of your drug candidate in-vivo.

Metabolite Identification

If detailed structural identification of metabolites is required, Novatia can utilize a variety of techniques such as: multi-stage MS/MS, high resolution MS, low-level component isolation and high-field capillary NMR analyses. One of our unique systems, called SepNMR, is designed to automatically isolate low-level components under conditions suitable for trace NMR analysis, thus lowering the burden for obtaining detailed structural information on critical samples. These powerful techniques can be used to answer questions ranging from biotransformation site of attachment to complete structural assignment on metabolites produced from in-vitro or in-vivo origin. The amount of detail obtained can be tailored to meet your specific project needs.

Please feel free to contact us for more information.