A direct area percent measurement of a UV chromatogram at a single wavelength is a quick, useful assessment of purity when comparing one sample to another.  However, it lacks specificity for measuring the actual quantity of any given substance [Figure 1].    A single determination of Relative Response Factors (RRFs) using Quantitative NMR (qNMR) allows for simple and accurate quantitation of impurities which eliminates the need for preparation, qualification, and storage of reference standards.  An example of RRF determination by qNMR is available in our white paper , which demonstrates quantitation of known impurities that have variable responses to UV-VIS detection, thereby providing a more accurate assessment of impurity levels than UV-VIS response alone.

ProMass for the Web is a charge deconvolution program that allows for interactive processing of ESI mass spectra of biomolecules. ProMass uses a novel charge deconvolution algorithm known as ZNova that produces artifact-free deconvoluted mass spectra. ZNova can be used to process data from a wide variety of biomolecules including proteins, oligonucleotides, peptides, etc.

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