Which Routine MS Analysis Should I Select?

Please select the options below that best match your sample and the type of data you are looking to obtain. Additional options will appear based on selections you make to help with determining a routine MS analysis that is best for your sample and analytical needs.

Unsure about selections?- Click Here

If you are unsure about the selections or have a sample that does not fit match the options provided below. Contact us using our Novatia “Contact Us” page, select “Questions about MS analysis” and provide us with as much information as you are able so that we can assist you in determining if a routine method is suitable or if we are able to assist with a custom quoted project.

Determined the method that best suits your sample?- Click Here

If you have determined a method that would be best suited for your sample(s) you can download our most recent blank routine sample submission sheet.

Download Blank Routine Sample Submission Sheet V3.4

We do not provide quotes for routine services, but if the Excel sheet is filled out correctly it will provide you with an accurate cost for the submission. Before sending samples to Novatia, be sure to upload the accurately filled out Excel sheet and complete an online sample submission.

Click Here to Visit Our Online Sample Submission Page!

Select Your Sample Type
Is your sample a Small molecule, Peptide/Protein/mAb, or Oligonucleotide
- Small Molecule
- Protein Based
- Nucleotide Based
Is this a peptide, peptide-nucleic acid (PNA), protein, monoclonal antibody (mAb) or antibody-drug-conjugate (ADC)?
Peptide, Peptide-Nucleic Acid, Protein, mAb or ADC?
- Peptide
- PNA
- Protein
- mAb
- ADC
**If the sample has a conjugated molecule and you require a DAR calculation, please follow the Antibody Drug Conjugate sample type.
Is your protein larger or smaller than 32 kDa?
Is your protein larger or smaller than 32 kDa?
- Protein is smaller than 32 kDa
- Protein is larger than 32 kDa
Do you require a DAR calculation?
Do you require a DAR calculation?
- Yes
- No
Is this a cysteine conjugated mAb?
Is this a cysteine conjugated mAb?
- Yes
- No
Do you want to observe the intact ADC?
Do you want to observe the intact ADC?
- Yes
- No
Do you require sequence confirmation of your protein by LC-MS/MS?
Do you require sequence confirmation of your protein by LC-MS/MS?
- Yes
- No
Do you require sample treatment such as reduction or PNGase treatment?
Do you require sample treatment such as reduction or PNGase treatment?
- Yes
- No
**Peptide-nucleic acids are considered peptides, please select protein-based sample type.
**For phosphoramadites please select small molecule as the sample
Is this a Synthetic Oligonucleotide or mRNA sample?
Is this a Synthetic Oligonucleotide or mRNA sample?
- Synthetic Oligonucleotide
- mRNA
Select the size range your oligonucleotide fits in:
Select the size range your oligonucleotide fits in:
- 5-40 nucleotides long
- 41-100 nucleotides long
- 101-400 nucleotides long
Is this a phosphoramidite?
Is this a phosphoramidite?
- Yes
- No
Do you require sequence confirmation by MS/MS?
Do you require sequence confirmation by MS/MS?
- Yes
- No
Do you require high resolution accurate mass data?
Do you require high resolution accurate mass data?
Do you require high resolution accurate mass data?
- Yes
- No
32k protein-Do you require high resolution accurate mass data?
- Yes
- No
High resolution accurate mass data will provide monoisotopic mass measurements that are < 3 ppm mass error from.
High resolution accurate mass data will provide monoisotopic mass measurements that are < 3 ppm mass error from.
Is this a cap or PolyA tail sample?
Is this a cap or PolyA tail sample?
- Capped mRNA sample
- PolyA tail
Do you require chromatographic separation for impurity profiling?
Do you require chromatographic separation for impurity profiling?
- Yes
- No
If the sample is heterogeneous we strongly recommend methods with chromatographic separations.
Do you require structure confirmation of the target species by MS/MS?
Do you require structure confirmation of the target species by MS/MS?
- Yes
- No
Recommended Method
Please be sure to answer all the above questions to populate a recommended method. This recommendation is based on your selections for generic questions. Reach out to us using our contact us page if you have any questions.

Routine MS Methods for Small Molecule Samples

Method selection can be found under sample type Small Molecule of the Excel sample submission sheet. Confirmation of an expected product, MW determination of major component(s), or overall purity assessment, includes analyst interpretation time.

Analysis (Excel Sample Submission Sheet Code)	Price Per Analysis	Method Details
HRMS_LCMS_SmMol_TEAB	$450	Purpose: Impurity profiling of NTPs, short oligonucleotides (≤ 3 bases) or phosphoramidites. LC Method: 20 minute gradient MS Method: High Resolution, Monoisotopic Mass.

Routine MS Methods for Small Molecule Samples

Analysis (Excel Sample Submission Sheet Code)	Price Per Analysis	Method Details
LCMS_TEAB	$300	Purpose: Impurity profiling of NTPs, short oligonucleotides (≤ 3 bases) or phosphoramidites. LC Method: 20 minute gradient MS Method: Nominal Mass

Routine MS Methods for Small Molecule Samples

Analysis (Excel Sample Submission Sheet Code)	Price Per Analysis	Method Details
LCMS	$400	Purpose: Impurity profiling of small molecules with MW < 1500Da. LC Method: Run 1: 10 minute gradient; Run 2: 10 minute gradient. MS Method: Run 1: Nominal Mass, Pos ESI; Run 2: Nominal Mass, Neg ESI

Routine MS Methods for Small Molecule Samples

Analysis (Excel Sample Submission Sheet Code)	Price Per Analysis	Method Details
MW_Confirmation_SmMol	$125	Purpose: MW confirmation of small molecules with MW < 1500Da. LC Method: Flow Injection MS Method: Nominal Mass

Routine MS Methods for Small Molecule Samples

Analysis (Excel Sample Submission Sheet Code)	Price Per Analysis	Method Details
HRMS_LCMS_structure	$1000	Purpose: MW, structure confirmation, and impurity profiling of a single small molecule target with MW < 1500Da. LC Method: Run 1: Flow Injection for Ionization confirmation; Run 2: 10 minute gradient. MS Method: Run 1: Nominal Mass; Run 2: High Resolution, Monoisotopic Mass, MSⁿ Fragmentation

Routine MS Methods for Small Molecule Samples

Analysis (Excel Sample Submission Sheet Code)	Price Per Analysis	Method Details
HRMS_LCMS_SmMol	$500	Purpose: Impurity profiling of small molecules with MW < 1500Da. LC Method: Run 1: Flow Injection for Ionization confirmation; Run 2: 10 minute gradient. MS Method: Run 1: Nominal Mass; Run 2: High Resolution, Monoisotopic Mass

Routine MS Methods for Small Molecule Samples

Analysis (Excel Sample Submission Sheet Code)	Price Per Analysis	Method Details
HRMS_structure	$750	Purpose: MW and structure confirmation of a single small molecule target with MW < 1500Da. LC Method: Flow Injection MS Method: High Resolution, Monoisotopic Mass, MSⁿ Fragmentation

Routine MS Methods for Small Molecule Samples

Analysis (Excel Sample Submission Sheet Code)	Price Per Analysis	Method Details
HRMS_SmMol	$300	Purpose: MW confirmation of small molecules with MW < 1500Da. LC Method: Flow Injection MS Method: High Resolution, Monoisotopic Mass

Routine MS Methods for mRNA Samples

Novatia does not perform sample prep to remove the 5' capped sequence or polyA tail from the larger portion of mRNA.
Visit our mRNA analysis page to view more details.

Analysis (excel sample submission sheet code)	Price Per Analysis	Method Details	Sample Treatment Done by Novatia	Additional Information needed
HRMS_LCMS	$350	Sample type: capped mRNA species between <40 bases LC method: 10-20 minute gradient (dependent on expected sequence) MS method: high resolution, monoisotopic mass	n/a	- Capping method - Type of cap - Modified bases - Expected sequence +1-2 nts - Method used to cleave mRNA
E1_digest_cap_HRMS_LCMS	$950	Sample type: capped mRNA (>50 uL of 1 mg/ml) LC method: 28 minute gradient MS method: high resolution, monoisotopic mass	mRNA digestion by Enzyme 1	- Capping method - Type of cap - Provide the first 5 nts of the mRNA sequence - Length of mRNA sequence
E1_digest_cap-tail_HRMS_LCMS	$1350	Sample type: mRNA with a 5' cap and 3' polyA tail <140 bases (>50 uL of 1 mg/ml) LC method: 28 minute gradient for cap analysis/ 20 minute gradient for polyA tail analysis MS method: high resolution, monoisotopic mass for cap analysis/ average mass for polyA tail analysis	mRNA digestion by Enzyme 1	- Capping method - Type of cap - Provide the first 5 nts of the mRNA sequence. - Length of mRNA sequence- Length of polyA tail - Disclose if there are any non-rA nts at the beginning of or interrupting the polyA tail

Routine MS Methods for mRNA Samples

Novatia does not perform sample prep to remove the 5' capped sequence or polyA tail from the larger portion of mRNA.
Visit our mRNA analysis page to view more details.

Analysis (excel sample submission sheet code)	Price Per Analysis	Method Details	Sample Treatment Done by Novatia	Additional Information needed
HRMS_LCMS_PolyAtail	$350	Sample type: Poly A tail portion of mRNA sample <140 bases LC method: 20 minute gradient MS method: average mass	n/a	- Length of polyA tail - Disclose if there are any non-rA nts at the beginning of or interrupting the polyA tail
E1_digest_tail_HRMS_LCMS	$500	Sample type: mRNA sample containing a polyA tail <140 bases (>50 uL of 1 mg/ml) LC method: 20 minute gradient MS method: average mass	mRNA digestion by Enzyme 1	- Length of polyA tail - Disclose if there are any non-rA nts at the beginning of or interrupting the polyA tail
E1_digest_cap-tail_HRMS_LCMS	$1350	Sample type: mRNA with a 5' cap and 3' polyA tail <140 bases (>50 uL of 1 mg/ml) LC method: 28 minute gradient for cap analysis/ 20 minute gradient for polyA tail analysis MS method: high resolution, monoisotopic mass for cap analysis/ average mass for polyA tail analysis	mRNA digestion by Enzyme 1	- Capping method - Type of cap - Provide the first 5 nts of the mRNA sequence. - Length of mRNA sequence- Length of polyA tail - Disclose if there are any non-rA nts at the beginning of or interrupting the polyA tail

Routine MS Methods for Oligonucleotide Samples

Available for both DNA and RNA oligonucleotides.

Analysis (excel sample submission sheet code)	Price Per Analysis	Method Details	Sample Treatment Done by Novatia	Control/Additional Information needed
Oligo_Sequencing	$1000 per oligo strand sequenced	Purpose: Sequence confirmation of oligos <40 nucleotides LC method: desalting only MS method: high resolution, monoisotopic mass	n/a	Must provide sequence
Oligo_Sequencing_Full_Report	$3000 per oligo strand sequenced	Purpose: Sequence confirmation with data interpretation and report for oligos <40 nucleotides LC method: desalting only MS method: high resolution, monoisotopic mass	n/a	Must provide sequence

Routine MS Methods for Oligonucleotide Samples

Available for both DNA and RNA oligonucleotides.

Analysis (excel sample submission sheet code)	Price Per Analysis	Method Details	Sample Treatment Done by Novatia	Control/Additional Information needed
HRMS	$150	Purpose: MW confirmation of oligos 5-100 nucleotides LC method: desalting only MS method: high resolution, monoisotopic mass	n/a	n/a

Routine MS Methods for Oligonucleotide Samples

Available for both DNA and RNA oligonucleotides.

Analysis (excel sample submission sheet code)	Price Per Analysis	Method Details	Sample Treatment Done by Novatia	Control/Additional Information needed
HRMS_5_min_LCMS	$250	Purpose: MW confirmation and impurity profiling of oligos 5-100 nucleotides LC method: 5-minute gradient MS method: high resolution, monoisotopic mass	n/a	n/a
HRMS_LCMS	$350	Purpose: MW confirmation and impurity profiling of oligos 5-100 nucleotides LC method: 20-minute gradient MS method: high resolution, monoisotopic mass	n/a	n/a

Routine MS Methods for Oligonucleotide Samples

Available for both DNA and RNA oligonucleotides.

Analysis (excel sample submission sheet code)	Price Per Analysis	Method Details	Sample Treatment Done by Novatia	Control/Additional Information needed
MW_confirmation	$100	Purpose: MW confirmation of oligos 5-100 nucleotides LC method: desalting only MS method: average mass	n/a	n/a

Routine MS Methods for Oligonucleotide Samples

Available for both DNA and RNA oligonucleotides.

Analysis (excel sample submission sheet code)	Price Per Analysis	Method Details	Sample Treatment Done by Novatia	Control/Additional Information needed
LCMS_5_min	$150	Purpose: MW confirmation and impurity profiling of oligos 5-400 nucleotides LC method: 5-minute gradient MS method: average mass	n/a	n/a
LCMS_20_min	$250	Purpose: MW confirmation and impurity profiling of oligos 5-400 nucleotides LC method: 20-minute gradient MS method: average mass	n/a	n/a