New Routine LC-MS Methods for mRNA Analysis!
Stop spending time optimizing cleavage of mRNA by RNAses before analysis and send us your intact mRNA sample! We have added new routine LC-MS methods for mRNA 5′ cap and polyA tail analysis that include a digestion done here at Novatia. Methods are available for 5′ cap analysis, polyA tail analysis, and the combination.
Routine MS Methods for mRNA Samples
Novatia does not perform sample prep to remove the 5' capped sequence or polyA tail from the larger portion of mRNA.Visit our mRNA analysis page to view more details.
| Analysis (excel sample submission sheet code) | Price Per Analysis | Method Details | Sample Treatment Done by Novatia | Additional Information needed |
|---|---|---|---|---|
| E1_digest_cap_HRMS_LCMS | $950 | Sample type: capped mRNA (>50 uL of 1 mg/ml) LC method: 28 minute gradient MS method: high resolution, monoisotopic mass | mRNA digestion by Enzyme 1 | - Capping method - Type of cap - Provide the first 5 nts of the mRNA sequence - Length of mRNA sequence |
| E1_digest_tail_HRMS_LCMS | $500 | Sample type: mRNA sample containing a polyA tail <140 bases (>50 uL of 1 mg/ml) LC method: 20 minute gradient MS method: average mass | mRNA digestion by Enzyme 1 | - Length of polyA tail - Disclose if there are any non-rA nts at the beginning of or interrupting the polyA tail |
| E1_digest_cap-tail_HRMS_LCMS | $1350 | Sample type: mRNA with a 5' cap and 3' polyA tail <140 bases (>50 uL of 1 mg/ml) LC method: 28 minute gradient for cap analysis/ 20 minute gradient for polyA tail analysis MS method: high resolution, monoisotopic mass for cap analysis/ average mass for polyA tail analysis | mRNA digestion by Enzyme 1 | - Capping method - Type of cap - Provide the first 5 nts of the mRNA sequence. - Length of mRNA sequence- Length of polyA tail - Disclose if there are any non-rA nts at the beginning of or interrupting the polyA tail |