Novatia Sample Submission Form
To submit an analysis request, begin by downloading a blank routine sample submission sheet. It is best to download the most recent version of the sheet as you will not be able to upload an older version. Open the blank sheet in Microsoft Excel and enter the sample information and details of the analysis requested for each sample to be submitted. The sample submission sheet will tabulate the analysis requests and calculate the estimated cost. Save the sheet and proceed with Steps 1 through 4. Select the Submit button at the bottom of the page and a confirmation will be presented summarizing the request with shipping instructions. A confirmation will also be emailed to the contact entered in Step 3. (Check your spam folder if you have not received a confirmation email)
- Rush Services available for 100% additional cost
- See Novatia website for detailed pricing information
NMR Sample Requirements:
- 1D Standard Analysis: >1 mg
- 1D Standard 13C Analysis: >50 mg
- 2D Standard Analysis: >10 mg
NMR Analysis Time:
- Analysis results usually within 5 business days of sample receipt
NMR Results:
- Standard PDF Plots with peak list and integration
- Raw data available upon request
NMR Experiment Key:
- Proton = Hydrogen
- F_19 = Fluorine
- P_31 = Phosphorus
- C_13 = Carbon
- C_13_DEPT = DEPT Carbon
- STAN_1H_COSY_HSQC_HMBC = 2D analysis including 1D proton, COSY, HSQC, and HMBC
- Rush Services available for 100% additional cost
- See Novatia website for detailed pricing information
Note: MW Confirmation refers to nominal mass only
MS Sample Requirements
- Minimum sample amounts
- Small Molecules
- 50-100 ug (e.g. 1-2 mg/mL x 25-50 uL)
- Ideal for small molecule routine work would be at least 50 microliters of 400 micromolar sample.
- Oligonucleotides
- Submit > 1 nmol if sending an oligonucleotide for oligo sequencing
- Submit > 50 pico moles for oligo analyses. An ideal oligo submission for routine work would be at least 30 microliters of 5 micromolar sample
- Proteins/ Peptides
- 50-100 ug (e.g. 2 mg/ mL x 25-50 uL) for peptide mapping
- 20-40 ug (e.g. 2 mg/mL x 10-20 uL) for Native MS methods
- 10-20 ug (e.g. 1 mg/mL x 10-20 uL) for all other protein/peptide analysis.
- Small Molecules
- Samples can be dry or lyophilized sample (specify grams or moles) or in solution (specify volume & concentration)
- If the samples contain any detergent (Tween, TritonX, etc) tell us in the comments and provide an estimated concentration.
- Provide target molecular weight, molecular formula, or structure.
- Oligonucleotide sequences (use IDT designation as specified at IDT Oligo Nomenclature) this includes sequence modifications if they are available. If the modification is unique please provide a molecular formula or structure.
- If submitting a small molecule, please indicate the structure and/or functional groups, a solvent, and any other important information in the Comments field of the Excel sheet
Plate Submission Notes:
- To qualify for plate pricing:
- Each sample in the plate must be at a concentration between 5 uM – 10 uM with a minimum of 100 uL of sample.
- The plate MUST be skirted.
- Example of an accepted 96-Well Plate: https://www.thermofisher.com/order/catalog/product/A31728
- To prevent cross-contamination and potential sample loss, it is best to ship them either on dry ice, or dried-down.
- Even the best plate seals it is likely that cross-contamination will happen during the shipping process.
- Specify the plate position for each sample in the excel sheet.
- To qualify for plate pricing:
MS Analysis Time:
- MW Confirmation usually within 1 business day of sample receipt
- Purity Profile usually within 2 business days of sample receipt
- High Resolution data usually within 1-5 business days of sample receipt
- Large quantities of samples may take longer
MS Results:
- Web-based ProMass report available at our secure data server
- Click here to see an example of a ProMass report
Do not use this form…go to Novatia’s GMP NMR Sample Submission.