Oligo HTCS

Introducing the first fully-automated systems for oligonucleotide LC/HRMS!

The oligo high throughput characterization system (HTCS) is the first exclusive LC-MS system to automate every aspect of oligonucleotide mass spectral analysis. Novatia has integrated instruments from Thermo Fisher Scientific to create a turnkey system for high-throughput as well as detailed accurate mass oligonucleotide LC-MS analysis. Oligo HTCS takes care of sample injection, desalting, HRMS detection, and data processing and reporting. Our package includes software, methods, consumables, training, and support that enables the user to perform expert-level analysis. With a sample throughput of ~2400 samples/day (~36 seconds per sample), there is no other LC-MS system package on the planet that can match it. Our ProMass software ties it all together, allowing you to process and communicate your results effortlessly.

Applications include:

Oligonucleotide manufacturing QC
DNA, RNA, therapeutics, diagnostics
Detailed LC-HRMS profiling for the identification of impurities, degradants, and metabolites
Purity determination via LC-MS and/or LC-UV detection

Color-coded sample plate view from 96-well oligonucleotide HRMS analysis. The green sample well A8 indicates desired product was found as a major component. Mass spectrum of a 120-mer oligo acquired with the Oligo HTCS, **Target Mass: 36262.0122 Da, Mass Error: 1.7ppm.**

Key Features

~2400 samples/day throughput
< 3ppm mass accuracy with 180k resolution
Analyzes oligos exceeding 120 bases
On-line desalting eliminates need for off-line sample processing and improves data quality
Switches automatically from high-throughput mass confirmation to detailed LCMS
One-click automated data processing with web-based results display
Detailed profiling mode provides complete information about impurities and sequence failures
ProMass software processes entire sample lists without user intervention. ASAP processing allows automated data processing after each sample is analyzed (i.e., real-time processing)
HTML, XML, and Excel results output

Exceptional Benefits

Analyze oligos that are difficult or impossible to analyze by MALDI-ToF, including long oligos (>120 bases), oligos with labile chemical moieties (e.g., BHQ’s), poly-T’s, hairpin oligos, etc.
Perfect for the QC of long oligos (e.g., long guide RNA strands)
Analyzes both DNA and RNA oligos, particularly useful for analysis of siRNA oligos, including duplex oligos containing high salt concentrations
Less expensive to operate (per sample) than high-throughput chip-based MALDI-ToF systems
Web-based results summary including color-coded sample browser viewable from any web-browser
Excel-based sample list import and results summary
XML output aides in capturing results for LIMS or database

What’s Included with the Oligo HTCS

The Oligo HTCS includes everything you need to characterize your oligos using either high-throughput or detailed LC/MS methods. We install the system, provide detailed training for your users, and guarantee your success with the application. As part of the package, we provide ready-to-run methods for both high-throughput analysis and detailed LC/UV/MS profiling of oligos.

**New** Orbitrap Exploris MX HRMS LC/MS system from Thermo Scientific
Standard Oligo HTCS Vanquish system utilizes Vanquish Flex dual pump UHPLC, Flex autosampler, and UV detector from Thermo Scientific
Novatia ProMass HR deconvolution oligonucleotide data processing software package
Instrument methods for on-line desalting using dual trap column desalting platform for maximum throughput: 2400 samples/day
Instrument methods for detailed LC/MS profiling analysis
Oligo HTCS applications pack containing a supply of analytical and desalting columns, buffer conditions, and method files
Optional Orbitrap BioPharma package improves results for long oligonucleotides
Installation, training, system documentation, and full application support including a one year warranty
Fully configurable to suit your analysis and budget requirements, including configurations for users with existing Thermo LC/MS systems

Want a Quote or have questions?
Email us at oligohtcs@enovatia.com or click on the bubble below to fill out our inquiry form:

Who uses the Oligo HTCS?
Oligo HTCS systems are in use world wide in DNA/RNA manufacturing and pharmaceutical research environments. Some of our users include:

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