Sample Submission

Note: MW Confirmation refers to nominal mass only MS Sample Requirements

• Submit > 1 nmol if sending an oligonucleotide for oligo sequencing, or > 50 pico moles for all other oligo analyses. An ideal oligo submission for routine work would be at least 30 microliters of 5 micromolar sample
• Submit 50-100 ug (e.g. 1mg/mL x 50-100 uL) for MAb analysis. Tell us in the comments if the samples contain any detergent (Tween, TritonX, etc).
• Sample may be dry (specify grams or moles) or in solution (specify volume & concentration)
• Provide target molecular weight or putative oligonucleotide sequence (use IDT designation as specified at IDT Oligo Nomenclature) including sequence modifications if available
• If submitting a small molecule, please indicate the structure and/or functional groups, a solvent, and any other important information in the Comments field of the Excel sheet

Plate Submissions:

• Please send well-plate submissions either on dry ice, or dried-down to prevent cross-contamination or loss of samples, etc.
• Please specify the plate position for each sample when filling out the excel sheet.
• The concentration MUST be uniform throughout the plate at an appropriate concentration (5uM or 50 picomoles).
• There MUST be at least 100uL of volume in each well of the plate.
• The plate MUST be skirted.
• Links to appropriate 96-Well Plate Examples
  • https://www.fishersci.com/shop/products/fisherbrand-96-well-deepwell-polypropylene-microplates-5/12566120
  • https://www.thermofisher.com/order/catalog/product/A31728

MS Analysis Time:

• Oligonucleotide MW Confirmation usually within 1 business day of sample receipt
• Purity Profile usually within 2 business days of sample receipt
• High Resolution data usually within 1-5 business days of sample receipt

MS Results:

• • • Web-based ProMass report available at our secure data server

NMR Sample Requirements:

• 1D Standard Analysis: >1 mg
• 1D Standard 13C Analysis: >50 mg
• 2D Standard Analysis: >10 mg

NMR Analysis Time:

• Analysis results usually within 5 business days of sample receipt

NMR Results:

• Standard PDF Plots with peak list and integration
• Raw data available upon request

NMR Experiment Key:

• Proton = Hydrogen
• F_19 = Fluorine
• P_31 = Phosphorus
• C_13 = Carbon
• C_13_DEPT = DEPT Carbon
• STAN_1H_COSY_HSQC_HMBC = 2D analysis including 1D proton, COSY, HSQC, and HMBC