Sample Submission

Note: MW Confirmation refers to nominal mass only MS Sample Requirements

• Submit > 1 nmol if sending an oligonucleotide for oligo sequencing, or > 50 pico moles for all other oligo analyses. An ideal oligo submission for routine work would be at least 30 microliters of 5 micromolar sample
• Submit 50-100 ug (e.g. 1mg/mL x 50-100 uL) for MAb analysis. Tell us in the comments if the samples contain any detergent (Tween, TritonX, etc).
• Sample may be dry (specify grams or moles) or in solution (specify volume & concentration)
• Provide target molecular weight or putative oligonucleotide sequence (use IDT designation as specified at IDT Oligo Nomenclature) including sequence modifications if available
• If submitting a small molecule, please indicate the structure and/or functional groups, a solvent, and any other important information in the Comments field of the Excel sheet

Plate Submissions:

• • Discount Pricing is only available for the following MS methods:

    1. Oligonucleotide MW_Confirmation
    2. Oligonucleotide 5min_LCMS

    To Qualify for Discount Pricing:

    • Samples MUST be in a skirted plate with conical/V-shape wells. See below for accepted plates.
    • Plate position for each sample must be specified in Excel submission sheet.
    • All samples must be at concentrations between 5 uM – 10 uM with a minimum of 100 uL of sample.
      • • If plate is shipped dried-down, 0.5-1 nanomole of sample is required
    • Uniform MS method selected for all samples on the submission sheet.

    If these requirements are not met, the submission will be placed on a HOLD and the contact for that submission will be reached out. We will confirm whether to proceed with the requested analysis with full pricing per sample.

    Plate Shipping & Accepted Plates Information:

    • Plates are subject to review to qualify for discount pricing when received. Example of accepted 96-Well Plates are below:
      • https://www.thermofisher.com/order/catalog/product/A31728
      • https://www.thermofisher.com/order/catalog/product/249943
      • https://www.waters.com/nextgen/us/en/shop/vials-containers–collection-plates/wat058957-96-well-sample-collection-plate-1-ml-50-pk.html 
      • https://www.waters.com/nextgen/us/en/shop/vials-containers–collection-plates/186005837-96-well-sample-collection-plate-700–l-round-well-25-pk.html
    • If you are not using one of the plates listed above, we recommend you contact us to confirm if your plate is acceptable for discount pricing prior to submitting.
    • To prevent cross-contamination and potential sample loss, it is best to ship them either on dry ice, or dried-down.
      • • Even the best plate seals it is likely that cross-contamination will happen during the shipping process.

MS Analysis Time:

• Oligonucleotide MW Confirmation usually within 1 business day of sample receipt
• Purity Profile usually within 2 business days of sample receipt
• High Resolution data usually within 1-5 business days of sample receipt

MS Results:

• • • Web-based ProMass report available at our secure data server

NMR Sample Requirements:

• 1D Standard Analysis: >1 mg
• 1D Standard 13C Analysis: >50 mg
• 2D Standard Analysis: >10 mg

NMR Analysis Time:

• Analysis results usually within 5 business days of sample receipt

NMR Results:

• Standard PDF Plots with peak list and integration
• Raw data available upon request

NMR Experiment Key:

• Proton = Hydrogen
• F_19 = Fluorine
• P_31 = Phosphorus
• C_13 = Carbon
• C_13_DEPT = DEPT Carbon
• STAN_1H_COSY_HSQC_HMBC = 2D analysis including 1D proton, COSY, HSQC, and HMBC